■ 基本信息
啟動(dòng)子: | CMV |
復(fù)制子: | pUC |
終止子: | BGH poly（A）signal |
質(zhì)粒分類: | 哺乳細(xì)胞載體;蛋白過(guò)表達(dá)質(zhì)粒 |
質(zhì)粒大小: | 5506bp |
質(zhì)粒標(biāo)簽: | His Tag (6x), V5 Epitope Tag |
原核抗性: | Amp |
真核抗性: | G418 |
克隆菌株: | DH5a |
培養(yǎng)條件: | 37℃，有氧，LB |
表達(dá)宿主: | 哺乳細(xì)胞 |
誘導(dǎo)方式: | IPTG或乳糖及其類似物 |
5'測(cè)序引物: | T7 Fwd:5d[TAATACGACTCACTATAGGG]3 |
3'測(cè)序引物: | 根據(jù)序列設(shè)計(jì) |
■ 質(zhì)粒屬性
質(zhì)粒宿主: | 哺乳細(xì)胞 |
質(zhì)粒用途: | 蛋白表達(dá) |
片段類型: | ORF
|
片段物種: | 空載體
|
原核抗性: | Amp |
真核抗性: | G418 |
熒光標(biāo)記: |
|
■ 質(zhì)粒簡(jiǎn)介
All pcDNA? vectors contain a strong promoter for high-level expression in mammalian cells, a choice of selection marker for generating stable cell lines, and an epitope tag for easy detection with a monoclonal antibody and rapid purification on nickel-chelating resin. Each vector is available in three reading frames to simplify cloning in-frame with the fusion tag.
質(zhì)粒只保證關(guān)鍵序列正確，不保證表達(dá)效果。
■ 質(zhì)粒圖譜
■ 質(zhì)粒序列
質(zhì)粒序列請(qǐng)下載：
ZK361pCDNA3.1-V5-HisB哺乳表達(dá)質(zhì)粒.txt
