■ 基本信息
別名: | pCDNA3 |
啟動(dòng)子: | CMV |
復(fù)制子: | pUC |
終止子: | bGH poly(A) signal |
質(zhì)粒分類: | 哺乳系列質(zhì)粒；哺乳表達(dá)質(zhì)粒；pCDNA系列質(zhì)粒 |
質(zhì)粒大小: | 5446bp |
質(zhì)粒標(biāo)簽: | 無(wú) |
原核抗性: | Amp |
真核抗性: | G418 |
克隆菌株: | DH5a |
培養(yǎng)條件: | 37℃，5%CO2 |
表達(dá)宿主: | 293T等哺乳細(xì)胞 |
誘導(dǎo)方式: | 無(wú)需誘導(dǎo) |
5'測(cè)序引物: | pCDNA3.1-F（CTAGAGAACCCACTGCTTAC） |
3'測(cè)序引物: | pCDNA3.1-R（TAGAAGGCACAGTCGAGG） |
備注: | 常用的哺乳細(xì)胞真核表達(dá)載體 |
■ 質(zhì)粒屬性
質(zhì)粒宿主: | 哺乳細(xì)胞 |
質(zhì)粒用途: | 蛋白表達(dá) |
片段類型: | ORF
|
片段物種: | 空載體
|
原核抗性: | Amp |
真核抗性: | G418 |
熒光標(biāo)記: |
|
■ 質(zhì)粒簡(jiǎn)介
pCDNA3.0載體是專門設(shè)計(jì)用來(lái)在哺乳細(xì)胞中進(jìn)行高水平的穩(wěn)定表達(dá)和瞬時(shí)表達(dá)。其中人巨細(xì)胞病毒早期啟動(dòng)子（CMV）在廣泛的哺乳動(dòng)物細(xì)胞中可以實(shí)現(xiàn)高表達(dá)。另外為篩選穩(wěn)定細(xì)胞系提供了新霉素抗性基因，可用G418篩選。
pcDNA3.0 is 5.4 kb vectors designed for high-level stable and transient expression in mammalian hosts. High-level stable and non-replicative transient expression can be carried out in most mammalian cells. The vectors contain the following elements: Human cytomegalovirus immediate-early (CMV) promoter for high-level expression in a wide range of mammalian cells. Neomycin resistance gene for selection of stable cell lines.
pcDNA3.0 vector is designed for high-level, constitutive expression in a variety of mammalian cell lines. It contains a Geneticin selectable marker and a forward-orientation multiple cloning site. High-level stable and non-replicative transient expression can be carried out in most mammalian cells. The vectors contain the following elements: Human cytomegalovirus immediate-early (CMV) promoter for high-level expression in a wide range of mammalian cells Multiple cloning sites orientations to facilitate cloning .Neomycin resistance gene for selection of stable cell lines Episomal replication in cells lines that are latently infected with SV40 or that express the SV40 large T antigen.
特征片段：
CMV promoter: bases 209-863 T7 promoter: bases 864-882
Polylinker: bases 889-994 Sp6 promoter: bases 999-1016
BGH poly A: bases 1018-1249 SV40 promoter: bases 1790-2115
SV40 origin of replication: bases 1984-2069 Neomycin ORF: bases 2151-2945
SV40 poly A: bases 3000-3372 ColE1 origin: bases 3632-4305
Ampicillin ORF: bases 4450-5310
質(zhì)粒只保證關(guān)鍵序列正確，不保證表達(dá)效果。
■ 質(zhì)粒圖譜
■ 質(zhì)粒序列
質(zhì)粒序列請(qǐng)下載：
ZK344pCDNA3.0哺乳表達(dá)質(zhì)粒.txt
